Cytokeratin 19 Antibody
This antibody is specific to the rod domain of human Cytokeratin 19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. Cytokeratin 19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Cytokeratin 19 antibody reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Cytokeratin 19 antibody is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Human breast cancer MCF-7 cells were used as the immunogen for this Cytokeratin 19 antibody.
Clonality
Monoclonal
Isotype
IgG2a λ
Clone
A53-B/A2.26
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Cytokeratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cytokeratin 19 antibody is available for research use only.
Storage Conditions
Store the Cytokeratin 19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with Cytokeratin 19 antibody (clone A53-B/A2.26) . Predicted molecular weight ~43 kDa.
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