CD11c Antibody

CD11c, also known as Integrin alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Functional studies (order BSA/sodium azide-free format)

Host

Mouse

Reactivity

Human

Immunogen

Human monocytic U937 phorbol ester-treated cells were used as the immunogen for this CD11c antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HC1/1

Applications

FACS, IF, Functional studies

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD11c antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD11c antibody is available for research use only.

Storage Conditions

Store the CD11c antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11c antibody to be titered up or down for optimal performance.