CD11b Antibody / MAC-1 / ITGAM

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin alpha-M (CD11b) combines with the Integrin beta-2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3) . Integrin alpha-M/beta-2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Functional studies (order BSA/sodium azide-free format)

Host

Rat

Reactivity

Human, Mouse

Immunogen

B10 mouse spleen cells enriched for T lymphocytes were used as the immunogen for this CD11b antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

M1/70

Applications

FACS, IF, Functional studies

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD11b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD11b antibody is available for research use only.

Storage Conditions

Store the CD11b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11b antibody to be titered up or down for optimal performance.