Lambda Light Chain Antibody

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1-2 µg/mL

UniProt

P01701, P01842

Host

Mouse

Reactivity

Human

Immunogen

Purified human Ig was used as the immunogen for this Lambda Light Chain antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

LcN-2

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Lambda Light Chain antibody at 2-8oC, protected from light.

Limitations

This Lambda Light Chain antibody is available for research use only.

Storage Conditions

Store the Lambda Light Chain antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Lambda Light Chain antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, secreted

Image Legend

(Left) Immunofluorescent staining of permeabilized human Ramos cells with CF488-labeled Lambda Light Chain antibody (clone LcN-2, green) and DAPI (blue) . (Right) Negative control.