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CD50 Antibody

This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI) . It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format), Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

186-2G9

Applications

Blocking, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD50 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD50 antibody is available for research use only.

Storage Conditions

Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Membrane, cytoplasm

Image Legend

IHC analysis of FFPE human tonsil with CD50 antibody (clone 186-2G9) .

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