HSP60 Antibody / HSPD1

This antibody recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60) . The antibody's epitope is localized between amino acids 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's) . HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK2 antibody, unlike LK1 antibody, recognizes both mammalian and bacterial HSP60.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human HSP60 protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LK2

Applications

IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HSP60 antibody is available for research use only.

Storage Conditions

Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Mitochondria in cytoplasm

Image Legend

IHC staining of FFPE human liver tissue with HSP60 antibody (clone LK2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.