HSP60 Antibody / HSPD1
This antibody recognizes a 60 kDa protein, identified as the heat shock protein 60. The antibody's epitope is localized between amino acids 383-447 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's) . HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK1 antibody, unlike LK2 antibody, recognizes only the mammalian (not bacterial) HSP60 and is useful in distinguishing HSP60 from mammals and bacteria.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human HSP60 was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LK1
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HSP60 antibody is available for research use only.
Storage Conditions
Store the HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Mitochondria in cytoplasm
Image Legend
IHC testing of FFPE human breast carcinoma with HSP60 antibody (clone LK1) .
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