HSP27 Antibody
This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P04792
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
G3.1
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HSP27 antibody is available for research use only.
Storage Conditions
Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Predominantly cytoplasmic with some nuclear
Image Legend
IHC testing of FFPE human breast carcinoma (10X) stained with HSP27 antibody (clone G3.1) .
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