GFAP Antibody
This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
GFAP isolated from pig spinal cord was used as the immunogen.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GA-5
Applications
FACS, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the GFAP antibody at 2-8oC.
Limitations
This GFAP antibody is available for research use only.
Storage Conditions
Store the GFAP antibody at 2-8°C.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human cerebellum with GFAP antibody (clone GA-5) .
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