Ku70 + Ku80 Antibody PE Conjugate
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood, Immunofluorescence : 1:50-1:100 for 30 minutes at RT
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
KU729
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
PE Conjugate
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Ku70 + Ku80 antibody at 2-8oC. Conjugate is light sensitive, store in the dark.
Limitations
This Ku70 + Ku80 antibody is available for research use only.
Storage Conditions
Store the Ku70 + Ku80 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Location
Nuclear
Image Legend
FACS testing of human K562 cells with Ku70 + Ku80 antibody. Black=cells alone; Green=isotype control; Red= PE conjugated Ku70 + Ku80 antibody.
Frequently Asked Questions
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