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Ku70 + Ku80 Antibody

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100

Host

Mouse

Reactivity

Human

Immunogen

Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KU729

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Ku70 + Ku80 antibody at 2-8oC, protected from light.

Limitations

This Ku70 + Ku80 antibody is available for research use only.

Storage Conditions

Store the Ku70 + Ku80 antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Ku70 + Ku80 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= Ku70 + Ku80 antibody.

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