Ku70 + Ku80 Antibody

This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence/Immunocytochemistry: 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KU729

Applications

FACS, IF/ICC

Purity

Protein G purified monoclonal antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Ku70 + Ku80 antibody is available for research use only.

Storage Conditions

Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

Location

Nuclear

Image Legend

FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= AF488 conjugated Ku70 + Ku80 antibody.