Fibronectin Antibody

The HFN7.1 Fibronectin antibody is directed against the peptide core and reacts with both the plasma and cellular forms. The antibody blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

Purified human Fibronectin was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HFN7.1

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Fibronectin antibody is available for research use only.

Storage Conditions

Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titration of the antibody may be required for optimal performance.1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.