ER Antibody / Estrogen Receptor alpha

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL (1), Immunofluorescence: 1-3 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ER506

Applications

FACS, WB, IHC-P, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the ER antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ER antibody is available for research use only.

Storage Conditions

Store the ER antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.1. FFPE testing requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and cooling at RT for 20 minutes.