HER2 ErbB2 Antibody

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human HER2 protein was used as the immunogen for this antibody. (1) Its epitope is localized in the extracellular domain.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HRB2/282

Applications

ELISA, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the HER2 ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HER2 ErbB2 antibody is available for research use only.

Storage Conditions

Store the HER2 ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. This HER2 antibody binds to the extracellular/cell surface region of the protein.

Location

Extracellular/Intracellular cell membrane (this mAb is binds to the extracellular portion of the protein)

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using HER2 ErbB2 antibody (clone HRB2/282) . These results demonstrate the foremost specificity of the HRB2/282 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.