ErbB2 Antibody / HER2
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. C-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this ErbB2 antibody. Its epitope is localized in the extracellular domain.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
HRB2/258
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the ErbB2 antibody at 2-8oC, protected from light.
Limitations
This ErbB2 antibody is available for research use only.
Storage Conditions
Store the ErbB2 antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ErbB2 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of methanol-fixed human SLBR3 cells with ErbB2 antibody (clone HRB2/258, green) and Reddot nuclear stain (red) .
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