ErbB2 Antibody / HER2

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this ErbB2 antibody. (1)

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

HRB2/258

Applications

ELISA, FACS, IF

Purity

Protein G purified

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ErbB2 antibody is available for research use only.

Storage Conditions

Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.