ErbB2 Antibody / HER2
This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this ErbB2 antibody. (1)
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
HRB2/258
Applications
ELISA, FACS, IF
Purity
Protein G purified
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ErbB2 antibody is available for research use only.
Storage Conditions
Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Extracellular/Intracellular cell membrane
Image Legend
Immunofluorescent staining of methanol-fixed human SLBR3 cells with ErbB2 antibody (clone HRB2/258, green) and Reddot nuclear stain (red) .
Frequently Asked Questions
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