EGFR Antibody / EGF Receptor

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

GFR450

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

CF488 Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EGFR antibody at 2-8oC, protected from light.

Limitations

This EGFR antibody is available for research use only.

Storage Conditions

Store the EGFR antibody at 2-8°C, protected from light.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Image Legend

Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue) . Right: isotype control.