EGFR Antibody
This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
The immunizing protein for this antibody was recombinant human EGFR.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
GFR450
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGFR antibody is available for research use only.
Storage Conditions
Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cell surface
Image Legend
FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR antibody (green, clone GFR450) .
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
METTL10, NT (METTL10, C10orf138, Methyltransferase-like protein 10) (Azide free) (HRP) discontinued