GM-CSF Antibody

GM-CSF (Granulocyte/macrophage - Colony-stimulating factor) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL

Host

Rat

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

BVD2-21C11

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the GM-CSF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GM-CSF antibody is available for research use only.

Storage Conditions

Store the GM-CSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance.