P27Kip1 Antibody
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SX53G8
Applications
FACS, IF, IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p27Kip1 antibody is available for research use only.
Storage Conditions
Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone SX53G8, green) and Phalloidin (red) .
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