CDK1 Antibody / CDC2

This antibody recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

UniProt

P06493

Host

Mouse

Reactivity

Human

Immunogen

Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this CDK1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

POH-1

Applications

WB, IHC-P

Purity

Protein G purified antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CDK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDK1 antibody is available for research use only.

Storage Conditions

Store the CDK1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDK1 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.