HCAM Antibody / CD44
This antibody recognizes a cell surface glycoprotein of 80-95kDa, called CD44, or HCAM, on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V) . Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44-s) . Higher molecular weight isoforms are described in epithelial cells (CD44-v), which are believed to function in intercellular adhesion and stromal binding. HCAM antibody immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 2-4ug/10^6 cells, Immunofluorescence: 2-4 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this CD44 / HCAM antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
156-3C11
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HCAM antibody is available for research use only.
Storage Conditions
Store the HCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human HeLa cells with HCAM antibody (clone 156-3C11, green) and Reddot nuclear stain (red) .
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