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CD34 Antibody PE Conjugate

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood), Immunofluorescence: 1:50-1:100

Host

Mouse

Reactivity

Human, Rat

Immunogen

Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ICO-115

Applications

FACS

Purity

Protein G affinity chromatography

Format

PE Conjugate

Buffer

500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD34 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Limitations

This antibody is available for research use only.

Storage Conditions

Store the CD34 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Formulation

500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (ICO-115, red) and isotype control antibody (green) . The PPI-negative cell population was gated for analysis.
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