CD33 Antibody

This antibody recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505) . CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells

Host

Mouse

Reactivity

Human

Immunogen

Human AML cells were used as the immunogen for this CD33 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

WM53

Applications

FACS

Purity

Protein G purified monoclonal antibody

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD33 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD33 antibody is available for research use only.

Storage Conditions

Store the CD33 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD33 antibody to be titered up or down for optimal performance.1. Not suitable for use in FFPE IHC testing.