CD28 Antibody
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this CD28 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CB28
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD28 antibody at 2-8oC, protected from light.
Limitations
This CD28 antibody is available for research use only.
Storage Conditions
Store the CD28 antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD28 antibody should be determined by the researcher.
Image Legend
Flow cytometry staining of PFA-fixed human Jukat cells with unlabeled CD28 antibody (clone CB28) ; Red=isotype control, Blue= CD28 antibody.
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