CD2 Antibody

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Human thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

1E7E8.G4

Applications

Functional studies, FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD2 antibody is available for research use only.

Storage Conditions

Store the CD2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

Western blot testing of human Jurkat cell lysate with CD2 antibody (clone 1E7E8.G4) . Expected molecular weight: 38-50 kDa depending on level of glycosylation.