Cyclin A2 Antibody

This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Full length bovine protein was used as the immunogen for this CCNA2 antibody. The epitope has been localized to amino acids 144-148 of the human protein.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

E67

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Cyclin A2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cyclin A2 antibody is available for research use only.

Storage Conditions

Store the Cyclin A2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.