Bcl-X Antibody
This antibody recognizes a protein of 27kDa, identified as Bcl-X. The mAb shows no cross-reaction with Bcl2 alpha or Bax. Bcl-X has two isoforms, XL (long), a 241 amino acid protein which suppresses cell death, and XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids QSNRELVVDFLS (3-14) of the human protien were used as the immunogen for this Bcl-X antibody (2) .
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
BX006
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Bcl-X antibody is available for research use only.
Storage Conditions
Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic and cell/nuclear membrane
Image Legend
IHC testing of Hodgkin's lymphoma stained with Bcl-X antibody (BX006) . Note cytoplasmic and membrane staining.
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