Cyclin D1 Antibody PE Conjugate
This antibody recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression. The antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood, Immunofluorescence: 1:50-1:100 for 30 minutes at RT
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Immunogen
Human recombinant full length Cyclin D1 protein was used as the immunogen.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
DCS-6
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
PE Conjugate
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Limitations
This Cyclin D1 antibody is available for research use only.
Storage Conditions
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Location
Nuclear
Image Legend
FACS testing of Jurkat cells: Black=cells alone; Green=isotype control; Red=Cyclin D1 antibody
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