Smooth Muscle Actin Antibody
Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P62736
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth Muscle Actin antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
1A4
Applications
FACS, IF, IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Smooth Muscle Actin antibody at 2-8oC (aliquot/freeze the azide-free format).
Limitations
This Smooth Muscle Actin antibody is available for research use only.
Storage Conditions
Store the Smooth Muscle Actin antibody at 2-8°C (aliquot/freeze the azide-free format) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of fixed human HeLa cells with Smooth Muscle Actin antibody (clone 1A4, green) and NucSpot nuclear stain (red) .
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