ZNF609 Antibody / KIAA0295

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. zinc finger protein 609 (ZNF609) is a 1,411 amino acid member of the Kruppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF609 contains one C2H2-type zinc finger through which it is thought to be involved in DNA-binding and transcriptional regulation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O15014

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R1122-R1411) was used as the immunogen for the ZNF609 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ZNF609 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ZNF609 antibody is available for research use only.

Storage Conditions

After reconstitution, the ZNF609 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ZNF609 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human Caco-2 cells with ZNF609 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ZNF609 antibody.