GFPT1 Antibody / GFAT

Glucosamine--fructose-6-phosphate aminotransferase isomerizing 1 is an enzyme that in humans is encoded by the GFPT1 gene. This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q06210

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids RDHTYAKCQNALQQVVAR from the human protein were used as the immunogen for the GFPT1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the GFPT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GFPT1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GFPT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GFPT1 antibody should be determined by the researcher.

Location

Cytoplasmic, extracellular

Image Legend

IHC staining of FFPE human splenic rupture tissue with GFPT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.