IRE2 Antibody / ERN2

IRE2/ERN2 induces translational repression through 28S ribosomal RNA cleavage in response to endoplasmic reticulum (ER) stress. This pro-apoptotic appears to play no role in the unfolded-protein response, unlike closely related proteins. Overexpression of IRE2/ERN2 activates both BiP and CHOP expression, and also leads to the development of programmed cell death. It has been suggested that Ern2 plays a role in multiple facets of the ER stress response in mammalian cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q76MJ5

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids K517-P919) was used as the immunogen for the IRE2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the IRE2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IRE2 antibody is available for research use only.

Storage Conditions

After reconstitution, the IRE2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the IRE2 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human Jurkat cells with IRE2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= IRE2 antibody.