Clgn Antibody / Calmegin

Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P52194

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Recombinant mouse protein (amino acids N18-D611) was used as the immunogen for the Clgn antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Clgn antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Clgn antibody is available for research use only.

Storage Conditions

After reconstitution, the Clgn antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Clgn antibody should be determined by the researcher.

Location

Cytoplasmic (ER)

Image Legend

Western blot testing of 1) rat testis, 2) rat H9c2 (2-1) cells and 3) mouse testis tissue lysate with Clgn antibody. Predicted molecular weight ~70 kDa but can be observed at ~93 kDa with an ~101 kDa phosphorylated form.