CIP4 Antibody / TRIP10

Cdc42-interacting protein 4 is a protein that in humans is encoded by the TRIP10 gene. TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2) . Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q15642

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein (amino acids M1-M182) was used as the immunogen for the CIP4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CIP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CIP4 antibody is available for research use only.

Storage Conditions

After reconstitution, the CIP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CIP4 antibody should be determined by the researcher.

Location

Cytoplasmic, cell membrane

Image Legend

Immunofluorescent staining of FFPE human A431 cells with CIP4 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.