Telomeric repeat-binding factor 1 Antibody / TRF1 / TERF1

Telomeric repeat-binding factor 1 is a protein that in humans is encoded by the TERF1 gene. This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P54274

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids Q119-R341) was used as the immunogen for the Telomeric repeat-binding factor 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Telomeric repeat-binding factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Telomeric repeat-binding factor 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Telomeric repeat-binding factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Telomeric repeat-binding factor 1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human A431 cells with Telomeric repeat-binding factor 1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.