Pyruvate Kinase Antibody / OIP-3 / PKM2

PKM (Pyruvate Kinase, Muscle), also known as OIP-3, PK3 and PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2) . By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P14618

Host

Mouse

Reactivity

Human

Immunogen

N-terminal region amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR from the human protein were used as the immunogen for the Pyruvate Kinase antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

11I4C3

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Pyruvate Kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Pyruvate Kinase antibody is available for research use only.

Storage Conditions

After reconstitution, the Pyruvate Kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Pyruvate Kinase antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with Pyruvate Kinase antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.