NF1 Antibody / Neurofibromin

Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P21359

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids R160-Q270) was used as the immunogen for the NF1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

4F8B7

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NF1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NF1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human T-47D cells with NF1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.