ACSL4 Antibody / FACL4

Long-chain-fatty-acid--CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

O60488

Host

Mouse

Reactivity

Human

Immunogen

Amino acids EPWTPETGLVTDAFKLKRKELR from the human protein were used as the immunogen for the ACSL4 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

4I7

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the ACSL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ACSL4 antibody is available for research use only.

Storage Conditions

After reconstitution, the ACSL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ACSL4 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE moderately differentiated adenocarcinoma of human rectum tissue with ACSL4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.