Prothrombin Antibody

F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1) . Thrombin also inhibits coagulation by activating protein C.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P00734

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids Y97-R124) was used as the immunogen for the Prothrombin antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

4G13G9

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Prothrombin antibody is available for research use only.

Storage Conditions

After reconstitution, the Prothrombin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Prothrombin antibody should be determined by the researcher.

Location

Secreted

Image Legend

IHC staining of FFPE human ovarian cancer tissue with Prothrombin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.