EPRS1 Antibody / Proline--tRNA ligase

Bifunctional aminoacyl-tRNA synthetase is an enzyme that in humans is encoded by the EPRS gene. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P07814

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R1298-Y1512) was used as the immunogen for the EPRS1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

12B5B3

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the EPRS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EPRS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the EPRS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EPRS1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Flow cytometry testing of human Caco-2 cells with EPRS1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= EPRS1 antibody.