GLS Antibody / Glutaminase
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Product Specifications
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O94925
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids K396-N654) was used as the immunogen for the GLS antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
3G13
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This GLS antibody is available for research use only.
Storage Conditions
Product Datasheet
https://www.nsjbio.com/tds-pdf/gls-antibody-glutaminase-3g13-rq6734
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the GLS antibody should be determined by the researcher.
CAS Number
9007-83-4
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human breast cancer tissue with GLS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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