Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1

Aldehyde dehydrogenase 1 family, member A1, also known as ALDH1A1 or retinaldehyde dehydrogenase 1 (RALDH1), is an enzyme that in humans is encoded by the ALDH1A1 gene. It is mapped to 9q21.13. The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P00352

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids T6-Q342) was used as the immunogen for the Aldehyde Dehydrogenase 1A1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

4C3

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Aldehyde Dehydrogenase 1A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Aldehyde Dehydrogenase 1A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Aldehyde Dehydrogenase 1A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Aldehyde Dehydrogenase 1A1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Flow cytometry testing of human HepG2 cells with Aldehyde Dehydrogenase 1A1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Aldehyde Dehydrogenase 1A1 antibody.