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NSD3 Antibody / Nuclear SET domain-containing protein 3

Histone-lysine N-methyltransferase NSD3 is an enzyme that in humans is encoded by the WHSC1L1 gene. This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9BZ95

Host

Rabbit

Reactivity

Human, Monkey

Immunogen

Recombinant human protein (amino acids Q46-K1425) was used as the immunogen for the NSD3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NSD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NSD3 antibody is available for research use only.

Storage Conditions

After reconstitution, the NSD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NSD3 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human A549, 2) monkey COS-7, 3) human HeLa and 4) human MCF7 cell lysate with NSD3 antibody. Predicted molecular weight: 155-162 kDa (long forms) and ~ 73 kDa (short form) .

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