EEF2K Antibody / Eukaryotic elongation factor 2 kinase

Eukaryotic elongation factor-2 kinase (eEF-2 kinase or eEF-2K), also known as calmodulin-dependent protein kinase III (CAMKIII) and calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, is an enzyme that in humans is encoded by the EEF2K gene. This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O00418

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein (amino acids K162-A719) was used as the immunogen for the EEF2K antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the EEF2K antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EEF2K antibody is available for research use only.

Storage Conditions

After reconstitution, the EEF2K antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EEF2K antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Immunofluorescent staining of FFPE human HepG2 cells with EEF2K antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.