GSPT1/2 Antibody / eRF3A/B

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A/B is an enzyme that in humans is encoded by the GSPT1/2 genes. This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P15170, Q8IYD1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids K138-D499) was used as the immunogen for the GSPT1/2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the GSPT1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GSPT1/2 antibody is available for research use only.

Storage Conditions

After reconstitution, the GSPT1/2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GSPT1/2 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human gastric adenocarcinoma tissue with GSPT1/2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.