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HES5 Antibody

Transcription factor HES-5 is a protein that in humans is encoded by the HES5 gene. This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q5TA89

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids M1-R121) was used as the immunogen for the HES5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HES5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HES5 antibody is available for research use only.

Storage Conditions

After reconstitution, the HES5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HES5 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human spleen tissue with HES5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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