Fcgr3 Antibody / Cd16

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcgR) . FcgR is divided into three classes, Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16) . CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcgRIIIa and CD16b/FcgRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P08508

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Recombinant mouse protein (amino acids A31-T215) was used as the immunogen for the Fcgr3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Fcgr3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Fcgr3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Fcgr3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Fcgr3 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of mouse RAW264.7 cells with Fcgr3 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Fcgr3 antibody.