5HT1B Antibody / HTR1B

5-hydroxytryptamine receptor 1B also known as the 5-HT1B receptor is a protein that in humans is encoded by the HTR1B gene. The protein encoded by this intronless gene is a G-protein coupled receptor for serotonin (5-hydroxytryptamine) . Ligand binding activates second messengers that inhibit the activity of adenylate cyclase and manage the release of serotonin, dopamine, and acetylcholine in the brain. The encoded protein may be involved in several neuropsychiatric disorders and therefore is often a target of antidepressant and other psychotherapeutic drugs.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P28222

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An E. coli-derived human protein (amino acids A35-S390) was used as the immunogen for the 5HT1B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the 5HT1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This 5HT1B antibody is available for research use only.

Storage Conditions

After reconstitution, the 5HT1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the 5HT1B antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

IHC staining of FFPE human rectal cancer tissue with 5HT1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.